Chinese Journal of Medical History / Zhong Hua Yi Shi Za Zhi

[This article belongs to Volume - 53, Issue - 04]

Abstract : Water stress is one of the abiotic factors expected as a risk in many crops, this stress causes damage to all types of plants, such as citrus growing, and this damage will affect production, as well as the quality of the crop yields; Obtaining a rootstock that resists and/or tolerates this constraint makes it possible to reduce this damage. The aim of this work is to test and compare the action of water stress in vitro provoked by polyethylene glycol, at successive concentrations on the friable calluses from citrus rootstocks, as well as their influence on calli growth, fresh weight, dry weight, humidity, relative water content, total sugar content, and proline content. calluses tested in vitro from rootstock genotypes of which: Citrange Carrizo; Citrumelo 4475; citrus Volkameriana and Poncirus trifoliata, induced in Murashige and Tucker (1969) medium (MT medium), added with 6-Benzylaminopurine (BAP), and 2,4-dichloro phenoxy acetic acid (2,4D), before moving them to MT medium, add by successive concentrations of polyethylene glycol (PEG): 0%; 0,1%; 0,3%;0,6%, during 60 days. The results of this work, prove that the water stress provoked in vitro has a significant effect on all the parameters studied, this effect results in a decrease in the growth of calli, fresh weight, and dry weight of the calli tested, compared to those of control; On the other hand, the effect of water stress is expressed by an increase in osmolytes including the content of soluble sugars, and the content of proline, and also by a decrease in humidity and the relative content of water in stressed calli, compared to control calli. The increase in these osmolytes in the calluses tested could be an indicator of calli tolerance towards water stress.