Chinese Journal of Medical History / Zhong Hua Yi Shi Za Zhi

[This article belongs to Volume - 53, Issue - 05]

Abstract : Autism is a part of the group of pervasive developmental disorders (PDDs) characterized by disorders of social reciprocity; language and communication disorders with a restricted and stereotyped repertoire of interests and activities (ICD-10).To explore the effect of valproic acid (VAP) on various behavioral impairments and histo-biochemical markers in VPA-induced autism-like, the male rats were divided into two groups: Control group (T): received Ip saline solution (NaCl 9%) and treated group (VAP): received Ip valproic acid at 500mg/kg. Behavioral impairment was measured by different paradigms to evaluate social interaction, memory and anxiety. Then, the brain, and liver were removed for biochemical and histological examinations. Our results had shown that, prenatal exposure to VPA at day 12.5 had long-term effects on postnatal behavior in rats, notably, a significant reduction of social interactions, memory, and an increase of in anxiety. The oxidative markers (MDA & CAT) were altered, expressed by an increase of MDA level in various brain tissues including the prefrontal cortex, and hippocampus whereas; the enzymatic antioxidants activity of (CAT) in the same areas was depleted. Widespread abnormalities in the brain and liver structure were observed at viable cell levels (pyramidal neurons and Purkinje cells, hepatocytes). In conclusion, the results suggest that in-utero VPA exposure causes abnormalities in the brain and liver structures (Purkinje cells and pyramidal neurons) that might be linked to oxidative stress. Furthermore, understanding the altered brain architecture involved in neurogenesis and the neurotransmission and its related behavior induced by VPA exposure is needed.