The current study aims to investigate different pharmacological activities of Thelypteris arida through in-vitro approaches that can be used for future drug development. We have chosen an experimental design for our study and have been conducted in Department of botany, University of Chittagong, Bangladesh in May, 2023. Study was conducted after getting ethical research approval from the institution. The used stud protocol was to find out exact plant, Collection, Drying, Grinding the leaves of Thelypteris arida and major Methanolic Extract of Thelypteris arida. Data were collected from animals and plant. Study findings have shown that the Thelypteris Arida effective in treating a variety of diseases, including Malaria, Tuberculosis, and Cancer. Using Thelypteris Arida can use for biofuel production, environmental remediation, and in the rainforest ecosystem. Overall, thelypteris arida is a promising plant with a wide range of potential applications. It was concluded that in recent years, there has been a growing interest in Thelypteris Arida research, and it has the potential to be used for a variety of purposes, including medicinal purposes like Antioxidant, Anti- Inflammatory, anti-diabetic, Thrombolytic, Anthelmintic, Cytotoxic activity. Future research on thelypteris arida will be lead to new and innovative ways to use this plant to benefit society.