Bioelectrical impedance analysis (BIA) is an easy tool to assess total body water (TBW), from which body composition can be estimated using statistical equations. Never the less, standard BIA equations have not been sufficiently validated during HIV infection in Morocco’s population. We compared TBW estimates from multifrequency BIA with those from the reference method deuterium isotope dilution (Deut) in a cohort of 30 HIV-infected. We enrolled 30 HIV-infected. This study was conducted in collaboration between Unit of Clinical Nutrition – HMIMV V Rabat; ALCS and Unit in Nutrition and Food, URAC 39 Ibn Tofaïl University & Regional Designated Center of Nutrition (AFRA/IAEA). We measured anthropometry, TBWBIA, and TBWDeut. lHIV+. The average in total body water TBWBIA, and TBWDeut are respectively in kg [47,057± 8,752] and [43,773±5,629]. Bland-Altman’s analysis shows a coefficient of correlation Pearson (Average (EAU % BIA + TBW% D20)/2 x Difference (TBW% D20 - EAU % BIA)) is 0,2882 with an IC (95%) [- 0,0875 ;0,5922], the error 5,5898 and Biais is 2,0611 with interval confidence [-13,017; 8,895]. The validity of current BIA to assess TBW during HIV infection remains uncertain and needs more experience. To develop the equation we need to extend the population of study.